Layton receives a letter from the famed opera singer, Jenis Quatlane, inviting them to see her newest performance. She also asks Layton, Luke and Remi for assistance in a mystery, saying her friend Melina died one year ago, but returned later as a seven year old girl saying she had found the secret of eternal life, and she also knew all the secrets that only the true Melina could know. Naturally, the case fascinates Layton and his apprentice, so they head to the Crown Petone opera house, but Remi stays in London, searching for some girls that went missing in there.